Next-Generation Biotech Technology

Numerous labs have found VLI standard proteins to be a great source for experimental tools. The variety of VLI's stable isotope labeling protocols, the stability of their samples, and more importantly the robustness of their samples have provided new data in the solid state NMR community. Using VLI standards, the Baldus at Gottingen, Germany, developed a 13CP MAS spectrum of a hydrated U-[13C, 15N] labeled sample (recorded on a 600 MHz WB NMR spectrometer using TPPM2 proton decoupling.)

Some labs and scientists, including Dr. Kurt Zilm at Yale have used VLI labeled ubiquitin to develop tiny crystalline proteins that have allowed for the development of new methodology. The spectra from crystalline ubiquitin sample preparations provide more details than the powder x-ray diffraction of other ssNMR. The microcrystalline method is superior to the lyophilized sample since sample molecules are locally ordered, have low conductivity and are stable to temperature variations.

A variety of VLI labeled NMR standard proteins are available for methods development and the establishment of new protocols. Please contact us for special sample preparation.